Human leukocyte exastase ELISA Kit - 96tests

Quantitative Sandwich ELISA for Detection of Human Neutrophil Elastase in Serum, Plasma, and Biological Fluids

• Species Reactivity: Human (Homo sapiens)
• Target Protein: Leukocyte Elastase (also known as Neutrophil Elastase, ELA2)
• Abbreviation: HLE
• Alternative Names: ELA2, ELANE, ELA, PMN elastase, Neutrophil elastase
• Format: 96 Wells (Other quantities available. Please inquire.)
• Sample Types: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenates
• Detection Method: Sandwich ELISA
• Assay Type: Quantitative Immunoassay
• High Sensitivity and Specificity for HLE
• Ready-to-Use 96-Well Strip Plate Format
• Low Intra-/Inter-Assay CVs for consistent results
• Quantitative Colorimetric Detection (450 nm)
• Broad Sample Compatibility – Human serum, plasma, cell culture media
• Storage: 2–8°C | Shelf life: 12 months from manufacture

The Human Leukocyte Elastase ELISA Kit is a sensitive and specific immunoassay for the quantitative detection of human leukocyte elastase (HLE) in various biological fluids. HLE is a serine protease released from neutrophils and macrophages during inflammation and is involved in host defense, tissue remodeling, and inflammatory response regulation.

This kit is optimized for high-throughput screening and biomarker studies in conditions like COPD, cystic fibrosis, rheumatoid arthritis, sepsis, and cardiovascular inflammation.

Test Principle

This kit employs a sandwich ELISA format :

1. A capture antibody specific for human leukocyte elastase is pre-coated on the plate.
2. Standards and samples are added; elastase binds to the capture antibody.
3. A biotin-conjugated detection antibody is added, followed by streptavidin-HRP.
4. TMB substrate is added and a measurable color develops.
5. Absorbance is read at 450 nm, and HLE concentrations are calculated from the standard curve.