Sequencing is a crucial technique in molecular biology used to determine the exact order of nucleotides in a DNA molecule. This process plays a pivotal role in genetic research, diagnostics, and understanding biological mechanisms. The most common methods of sequencing include Sanger sequencing, a traditional approach, and Next-Generation Sequencing (NGS), which allows for high-throughput analysis of DNA. NGS enables researchers to sequence entire genomes rapidly and at a lower cost, making it ideal for applications such as cancer research, genetic diagnostics, and evolutionary studies. Advances in sequencing technologies continue to revolutionize personalized medicine, allowing for more precise and targeted treatments based on an individual's genetic makeup.